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| Research Focus & Application |
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I study how axon-targeted syntaphilin (SNPH) docks mitochondria in axons. To explore potential docking sites for SNPH, I examine the distribution of the cytoskeleton in axons using multicolor fluorescence. These images show the localization of actin filaments, microtubules, and neurofilaments within axons.
| Microscopy and Imaging Methods |
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I use the LSM 510 Meta, which can visualize multiple fluorophores and allows me to examine the distribution of different proteins within my cross sections. | | Rat sciatic nerve fibers were fixed, sliced cross-sectionally, and stained with phalloidin (green) for actin filaments as well as with antibodies for tubulin (blue), and neurofilament (red). |