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| Microscopy and Imaging Methods: |
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A Z-stack comprised of 262 optical sections was collected using a 25X /0.8 LD LCI Plan-Apochromat objective using glycerin as immersion medium at an interval of 1.4 µm with 1024x1024 frame size and a XY pixel size of 330 nm. The images were collected using a 488 nm Argon laser with a pixel dwell time of 1.27 µs and the emission light was collected between 595-666 nm using a MBS 488. The maximum intensity projection image of the z-stack was produced using the Zen software supplied with the LSM 710. | | A confocal z-stack maximum intensity projection of an Arabidopsis thaliana anther (left) and part of a petal (right) labeled with periodic acid and propidium iodide that has been cleared and mounted in Hoyer’s solution containing chloral hydrate. |