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| Research Focus & Application |
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Research in the laboratory of Roger Tsien at the UCSD is focused on developing new chemical and biomolecular sensors to advance the imaging of biological processes at the ultrastructural, cellular, and organismal levels.
| Microscopy and Imaging Methods |
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Fly larvae or adult fly brain explants are imaged for GFP reporters marking neurons of interest. In some experiments, neurons will also express fluorescent reporters for newly synthesized proteins of interest. Our work involves obtaining large numbers of 3D images from both live and fixed specimens, using ever-changing sets of fluorophores. The speed and flexibility of the Axiovert 200 coupled with a LSM 5 Live system is a great benefit to our work. | | A projection series rotating around the Y axis constructed from a confocal z-stack of a fly larvae mouth revealing EGFP-labeled neurons. |