| |
Spinning Disk microscopes project the excitation light through hundreds of circular apertures simultaneously into the sample and collect the returning emission through the same confocal apertures. The beams are not actually scanned but moved by spinning the disk rapidly to illuminate the whole field of view.
 |
Zebrafish embryo development, maximum intensity projection from a single timepoint of a 10h timelapse. 25x/0.8 LD LCI Plan APOCHROMAT,
Tg(-8.0cldnb:lynGFP) (green) for membrane staining,nuclei are labeled with mCherry (red). J. Otte, M. Takamiya and U. Strähle, Karlsruhe Institute of Technology, Germany. |
| |
Out-of-focus light is almost entirely rejected by the disk, meaning the acquired image is already optically sectioned without the need for software processing. Spinning Disk systems are a form of parallel confocal microscope, meaning they work on multiple areas of the sample at the same time to trade a little resolution for a lot of speed.
Product:
Cell Observer® SD | |
|