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The technique called structured illumination projects a grid into the focal plane of a widefield fluorescent microscope. The grid is precisely moved in the lateral direction, and several images are acquired at the focal plane, each with the grid in a different position.
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| Rat hippocampus, triple fluorescence, maximum intensity projection of a 3D image stack, Plan APOCHROMAT 63x/1.4. Processed ApoTome.2 image. E. Fuchs and S. Bauch, DPZ Goettingen, Germany. |
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Since fluorescence emission from areas in focus varies more significantly per position than emission from out-of-focus areas, it is possible to automatically create an optical section free of out-of-focus blur. Whilst it takes a little more time to acquire the image than with the widefield deconvolution approach, this technique offers greater levels of out-of-focus discrimination.
Product:
ApoTome.2 | |
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