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| Single Point Laser Scanning |
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The most widely used form of optical sectioning microscope today is the spot scanner, a form of Laser Scanning Microscope (LSM). A single spot is focused into the sample and very rapidly moved across the field of view. The returning emission is filtered through a confocal pinhole aperture to reject the out-of-focus light.
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| Feather of an owl, Autofluorescence, depth coding. |
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The remaining light is detected by Photon Multiplier Tubes (PMTs) to form an image. The single spot approach is slower than parallel scanning techniques but offers great flexibility in terms of image size and imaging strategies, as well as the highest out-of-focus discrimination of all routine optical sectioning techniques.
Products:
LSM 700
LSM 710
LSM 780 | |
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