ZEISS Axioscan 7

일반 광학부터 형광, 편광 이미징까지 모두 가능한 슬라이드 스캐너

Digitize your specimens with Axioscan 7 – the reliable, reproducible way to create high-quality virtual microscope slides. Axioscan 7 combines qualities that you would not expect to get in a slide scanner: high speed digitization and outstanding image quality plus an unrivaled variety of imaging modes are all available in a fully automated and easy to operate system.

The most challenging research tasks as well as your routine scanning applications are supported by powerful hardware and perfectly featured software. Capture virtual slides quickly with high-speed scanning, while retaining consistently high quality, whether you want to capture brightfield, fluorescence or polarized light images.

ZEISS Axioscan 7 System
하이라이트
이미징 모드
광원과 카메라
응용 분야
다운로드
문의

어플리케이션의 한계에서 벗어나 자유롭게 스캔하세요

  • Fast automated scanning of up to 100 slides in a single run
  • Robust scan performance for continuous 24/7 operation
  • Easy-to-create scan profiles with intuitive wizards
  • Rapid switching among fluorescence, brightfield, and polarization 
  • Sophisticated filter concept for demanding fluorescence imaging 
  • Perfect image quality thanks to advanced Axiocam cameras

365일 24시간 가능한 스캐닝

From an automated system, you expect absolute reliability for continuous operation. ZEISS Axioscan 7 repeatedly produces digitized slides at dramatically improved speed, thanks to hardware components designed for extended, uninterrupted operation. A fully motorized condenser, powerful light sources and sensitive cameras ensure 24/7 scan performance, whether you have many similar slides or mixed applications to process. Easily assigned scan profiles allow acquisition runs to be set up quickly. Axioscan 7 software is built to flawlessly process large amounts of raw data – in the range of several terabytes.

24/7 Scan Performance
Automated Application Flexibility

자동화된 어플리케이션 유동성

ZEISS Axioscan 7 allows rapid switching among fluorescence, brightfield and polarization scanning modes, with the highest quality fast and gentle imaging fully available to you in each of these modes. Benefit from contrast flexibility and high scan speed when digitizing HE-stained tissue samples or other brightfield applications. Fast filter wheels and a spectral range up to far red excitation light expand your fluorescence imaging capabilities – in combination with the new sample-preserving contrast method Transfer of Intensity Equation, multiplex imaging reaches a new level of performance. The combination of all imaging modes let you extract maximum sample information with a minimum of effort.

더욱 적극적인 이미지 분석

ZEN Software를 기반으로 한 슬라이드 스캐닝

ZEN Slidescan is integrated within the powerful ZEN imaging software universe, which provides access to numerous additional processing
and analysis functions. ZEN Connect, the ZEISS software for correlative microscopy, enables more advanced workflows – from automated slide scanning to detailed studies on other microscope systems. The established CZI data format opens the possibility to use additional third-party data analysis tools. With ZEN Data Storage and ZEN Data Explorer, you can access and share your scanned data from anywhere, at any time.

The Bigger Picture: Slide Scanning within the ZEN Environment
Automated slide scanning and detailed studies on other ZEISS microscope systems combined in a ZEN Connect project

Axioscan 7 이미징 모드

효율적이고 생산적인 형광 이미징 모드

Speed, gentle treatment and the optimal wavelength are critical when it comes to multispectral fluorescence imaging. Axioscan 7 employs swift and reproducible LED illumination, fast filter wheels, and a sophisticated filter concept to efficiently separate a broad range of fluorescence channels.

  • Shortest possible exposure times for maximum specimen protection
  • Fast switching between up to 9 fluorescence channels
  • Perfect spectral separation for advanced fluorescence multiplexing applications
  • Unparalleled information density without compromising data quality
Non-small cell lung cancer (NSCLC) tissue stained with UltiMapper I/O PD-L1 kit.
Non-small cell lung cancer (NSCLC) tissue stained with UltiMapper I/O PD-L1 kit.

빠른 속도의 다양한 명시야 이미징 모드

The newly designed condenser with its motorized modulator disk allows automatic switching between different brightfield imaging modes to adapt to the different requirements of your applications, opening a new range of experiments and modality combinations.

  • Dramatically improved scan speeds in all brightfield imaging modes
  • Better sample detection and focusing
  • New phase and relief contrast options
  • Circular and linear polarization now fully supported

Mouse kidney wound healing assay, stained with sirius red
Sample courtesy: Alexander Lomow, Evotec

Brightfield
Cross linear polarization
The Axioscan 7 brightfield imaging performance is driven by a motorized condenser and a powerful white light source:
The Axioscan 7 brightfield imaging performance is driven by a motorized condenser and a powerful white light source: 1) Motorized modulator disc, 2) Circular polarizer, 3) Motorized linear polarizer, 4) Motorized aperture diaphragm, 5) WL-LED light source

타일 자국이 남지 않는 새로운 형광 초점 방식, TIE Contrast

향상된 검출력, 더 정확한 포커싱, 더 명확한 이미징

Using the new contrast method Transfer of Intensity Equation (TIE) for contrast generation in transparent samples, you record the interaction of a narrow cone of light with your sample’s structures in three images: one in focus, and two out of focus above and below the focal plane. From these three images, the phase information for the central plane is automatically extracted. Continuous acquisition in the z dimension, in combination with flash illumination and GPU-based fast image processing, enables very fast delivery of the final contrast images. You can choose to present this as either phase contrast or DIC-like relief contrast.

Solanum tuberosum – potatoe starch, 20x Plan-Apochromat 0.8
Solanum tuberosum – potatoe starch, 20x Plan-Apochromat 0.8; A) TIE phase contrast, B) TIE relief contrast, C) Brightfield

TIE contrast is an excellent tool to aid your experiments when working with sensitive fluorescent dyes:

  • Detect transparent tissues with little to no contrast in regular brightfield mode.
  • Speed the subsequent fluorescence imaging process with very fast flash-based focusing.
  • Protect your sensitive dyes from bleaching during focusing by using the lowest light doses.
  • Bring your fluorescent labels into context easily by applying the additional contrast information.

광원과 카메라

뛰어난 일루미네이션

형광 이미징을 위한 광원

Choose between Colibri 7, the super-fast 7 wavelengths LED light source from ZEISS, or the white light LED light source X-Cite Xylis:

ZEISS Colibri 7

7 wavelengths LED light source

ZEISS Colibri 7 – 7 wavelengths LED light source

Colibri 7 operates with reproducible output power levels for each wavelength and therefore produces consistent quantitative data for all important dyes, fluorescent proteins and probes. Individual LEDs and integrated excitation filters make an additional filter wheel unnecessary and allow switching times of a few milliseconds between the color channels.

X-Cite Xylis

White light LED light source

X-Cite Xylis – White light LED light source

The use of X-Cite Xylis together with a fast excitation filter wheel enables long wavelength sample illumination up to 770 nm. In addition, the green gap – typically a problem with LED fluorescent light sources – is overcome and comparable to classic arc lamps in this spectral range.

진보된 카메라

더 완벽한 이미지 퀄리티를 위해

Axioscan 7 is equipped with the most advanced Peltier-cooled cameras from the ZEISS Axiocam portfolio to support your brightfield and fluorescence applications with state-of-the-art imaging performance.

Color camera Axiocam 705 color

We have implemented the latest color Axiocam, the 705 color CMOS camera. It offers 5-megapixel resolution with a 3.45 µm pixel size and very low noise. With 55 frames per second acquisition speed in Axioscan 7 and a large field of view, Axiocam 705 rapidly accomplishes your brightfield and polarization imaging tasks.

Fluorescence camera Axiocam 712 mono

Axiocam 712 mono is the perfect choice for your fluorescence imaging applications. It offers small pixels (3.45 µm), fully capturing the resolution potential of the high numerical aperture optics, and a very low readout noise. Use camera binning of 2x2 pixels for increased sensitivity.

Fluorescence camera Axiocam 712 mono and color camera Axiocam 705 color

어플리케이션

Axioscan 7 실제 이미징

Reproducible Image Quality

ZEISS Axioscan 7 offers reliably reproducible image quality, no matter if you repeat your imaging task after a day, a week, a month or on a different machine.

Paraffin-embedded mouse kidneys from healthy wildtype animals (12 weeks).
Paraffin-embedded mouse kidneys from healthy wildtype animals (12 weeks). Nephrin stained with Cy3. PCNA APC (FarRed) and DAPI as counterstaining. Imaged with 20× NA 0.8 objective.
Colon sample from a patient with Crohn’s disease, imaged with 20× NA 0.8 objective.
Colon sample from a patient with Crohn’s disease, imaged with 20× NA 0.8 objective. Green: Cox-1 in Tuft cells in the epithelium – the sensory cells of the gut – and cells in the lamina propria connective tissue. Red: CD 163 – a macrophage marker.
Pleurosigma angulatum – diatomes, 20x Plan-Apochromat 0.8; Left: Brightfield,
Right: TIE relief

Non-small Cell Lung Cancer (NSCLC) Tissue

The UltiMapper I/O PD-L1 kit from Ultivue addresses whether a tumor is “hot” or “cold” and responsive to immune checkpoint inhibition because of a high immune filtrate (hot), in contrast to tumors with low immune infiltrates called “cold tumors” or non-T-cell-inflamed cancers – by exploring multiple cell phenotypes such as cytotoxic immune cells (CD8), immunosuppressive macrophages (Markers CD68, PD-L1) or immune evading tumor cells (Markers CK, PD-L1).

NSCLC tissue stained with UltiMapper I/O PD-L1 kit.
NSCLC tissue stained with UltiMapper I/O PD-L1 kit.
Picture detail.
Picture detail.

다운로드

ZEISS Axioscan 7

제품 브로셔


광범위한 어플리케이션을 위한 슬라이드 스캐너


pages: 25
file size: 5120 kB

Download

문의하기

이미징 시스템 전문가들에게 궁금하신 점을 남겨주세요.