ZEISS Lattice Lightsheet 7
Living cell의 장시간 입체 이미징을 위한 최적의 솔루션
ZEISS Lattice Lightsheet 7 makes light sheet fluorescence microscopy available for live cell imaging at subcellular resolution – while also allowing you to use your standard sample carriers. With this automated, easy-to-use system, volumetric imaging of subcellular structures and dynamics over hours and days with best protection from photo damage becomes available to everyone. Discover the dynamics of life in unprecedented depth of detail – with the ease you never imagined possible!
Did you miss the ZEISS Lattice Lightsheet 7 launch event stream?
Watch the on-demand version here
Learn about the lattice light sheet principle and its advantages for 3D imaging of subcellular dynamics, get insights into the technique's development process, get introduced to ZEISS Lattice Lightsheet 7 and its features, and hear about first customer experiences and applications.
세포 이하 단위의 움직임을 발견하세요
- Amazingly Simple Access
Examine living specimens directly on your standard sample carriers
- Next to no Photo Damage
Watch the subcellular dynamics of life over hours and even days
- Near-isotropic Resolution
Reveal three-dimensional details in their true proportions
- High-speed Volumetric Imaging
Don’t miss an interesting event on your coverslip
- Auto-aligning System
Focus your full attention on your experiments
누구나 손쉽게 사용할 수 있는 Lattice Light Sheet Technology
The importance of gentle light sheet imaging at high resolution cannot be overestimated for the study of subcellular processes. With Lattice Lightsheet 7, ZEISS makes access to the benefits of this advanced technology amazingly simple. Without having to adapt your usual sample preparation, you can examine living specimens directly on the standard sample carriers you already use for confocal microscopy. Complex alignment processes are performed automatically in this system so that you can focus your full attention on your experiments.
광독성(phototoxicity)과 블리칭으로부터 벗어나세요
You want to watch the dynamics of life at subcellular resolution to study how the finest structures change over time. But your conventional imaging systems quickly reach their limits because they are too invasive and destroy what you are observing. Instead, ZEISS Lattice Lightsheet 7 provides lattice-structured light that automatically adapts to your sensitive samples, resulting in a massive reduction of photobleaching and phototoxicity, to allow your experiments to continue over hours and even days. The controlled incubation environment and an integrated auto-immersion mechanism enable unattended long-term experiments.
Video: LLC-PK1 cell undergoing mitosis. Cells are expressing H2B-mCherry (cyan) and α-Tubulin mEGFP (magenta), recording over a period of 25 hours.
빠른 속도의 입체(volumetric) 이미징
The extremely fast image acquisition of ZEISS Lattice Lightsheet 7 enables up to three volume scans per second. Dynamic imaging of full sample volumes with this high temporal resolution means no longer missing an interesting event on your coverslip. Near-isotropic resolution along the X, Y and Z axes gives you a three-dimensional image of your sample that reveals structural details in their true proportions. Fast laser switching allows for imaging using up to three colors practically simultaneously, with minimized color crosstalk.
Video: Cos 7 cells transiently transfected with CalnexinmEmerald and EB3-tdTomato. EB3 labels the growing ends of microtubules and is necessary for the regulation of microtubule dynamics. Calnexin is a protein of the ER where proteins are synthesized. Cells were imaged for 1.5 hrs every 80 secs, imaged volume: 175 × 120 × 70 μm³.
Lattice Light Sheet 현미경 이론
Light sheet microscopy in general (also called Gaussian light sheet microscopy) is well known for its gentle imaging conditions at superior imaging speed. The groundbreaking concept of decoupling excitation and detection allows illumination of only the part of the specimen that is in the focal plane of the detection objective lens. By moving the sheet with respect to the sample and recording one image per focal plane, you can acquire volumetric data without exposing the out-of-focus sample areas.
Lattice light sheet microscopy combines the advantages of light sheet microscopy with near-isotropic resolution in the confocal range. Advanced beam shaping technology creates lattice-shaped light sheets that are significantly thinner than standard Gaussian light sheets and thus provide increased resolution at comparable imaging speeds. The lattice structure of the light sheet is created using a Spatial Light Modulator (SLM), then projected onto the sample after passing scanners that dither the lattice structure to create a smooth light sheet.
ZEISS의 Lattice Light Sheet 현미경 기술
During the development of Lattice Lightsheet 7, ZEISS gave special attention to user-friendliness and compatibility with conventional sample preparation techniques. An inverse configuration is the most important prerequisite to allow the use of standard sample carriers for high-resolution microscopy. The challenges resulting from an inverse configuration are mainly refractive index mismatches as fluorescence is emitted from the sample, passes through aqueous cell culture media, a tilted glass coverslip and water immersion, then into the detection objective.
Unrivaled ZEISS Optics
Special ZEISS proprietary optical elements in the detection beam path compensate for refractive index mismatches and enable you to image samples as easily and quickly as with a confocal microscope.
표준 사이즈 샘플 캐리어 사용
Without having to adapt your usual sample preparation, you can examine living specimens directly on the sample carriers you already use for confocal microscopy. ZEISS Lattice Lightsheet 7 can be used with all standard sample carriers that come with a no. 1.5 coverslip for the bottom:
- 35 mm dishes
- Chamber slides
- Multi-well plates
빠르고 부드러운 샘플 로케이션
With the integrated transmission LEDs and oblique detection which provide a DIC-like contrast, you can easily locate your sample. Change from white to red transmission LEDs for more gentle illumination if necessary. And you can choose to include transmitted light illumination during long-term observations.
샘플 수평 자동 조정
Specifically designed for this system, the unique 5-axis stage not only allows movement along the X, Y and Z axes, but also tilting with the highest precision in X and Y, compensating for even the smallest deviations in carrier dimensions or sample position. Leveling your sample is done automatically, which relieves you of tedious manual procedures.
내부 광학 파츠 자동 정렬
For the best imaging results, the lattice light sheet must be adapted to each sample; therefore, ZEISS has implemented automatic alignment of all optical elements to eliminate time-consuming manual adjustments. The innovative design of the excitation beam path allows for rapidly changing laser lines without having to reprogram the SLM. This enables virtually simultaneous acquisition of multi-channel data sets so that you will not miss any events occurring in your sample.
오퍼레이터 없이도 장시간 실험 가능
An integrated incubation system provides long-term stability throughout varying environmental conditions. The microscope controls and monitors temperature, CO2 and O2 levels, and humidity automatically, to preserve the integrity of your sample throughout the experiments. The lid with glass window allows quick and easy access to the sample to facilitate its inspection during an experimental run.
Prime the system to release any air, then a supply of immersion media tailored to the needs of your experiments is released automatically. Replenishing the immersion media is software-controlled, so you don’t have to worry about interfering with image acquisition. The reservoir is protected from illumination to keep bacterial growth at bay. Objectives are shielded from immersion supply; hence they remain dry, even if excess immersion media is applied.
|Typical Application||Typical Samples||Tasks|
Live cell imaging
3D cell culture
Small evolving organisms
Lattice Lightsheet 7 실제 이미징
Lamin B1 in Action
Lamin B1 localizes to the nuclear envelope and is involved in disassembling and reforming the nuclear envelope during mitosis. The formation of so-called ‘nuclear invaginations’ has been reported frequently for many different cell types during mitotic events at different stages of the cell cycle. Nuclear invaginations can manifest as tubular structures that extend from the nuclear envelope and cross through the nucleus. Although these unique structures have been reported frequently, most research so far has been done with fixed cells. Consequently, the function of these structures is largely unknown even though plenty of hypotheses have been proposed.
This data set was recorded with a cell line from the Allen Institute for Cell Science in Seattle: human induced pluripotent stem cells which endogenously express mEGFP-tagged lamin B1 (AICS-0013). The overnight experiment was recorded for close to 8 hours with one volume imaged every 1.5 min. Cells going through mitosis can be observed throughout the whole duration. Formation and dynamics of nuclear invaginations can clearly be observed i n most of the cells, throughout the complete cell cycle.
Gentle illumination is crucial for imaging mitosis as this process is extremely delicate and light sensitive. To prevent replication of damaged DNA, cells arrest mitosis as soon as there is any damage from excitation light. The gentleness of Lattice Lightsheet 7 imaging and an extremely stable system is required for imaging mitotic events over longer time periods. Fast volumetric imaging in combination with near-isotropic resolution allows for looking at the sample from every angle and investigating unique subcellular structures in every detail. ZEISS Lattice Lightsheet 7 is the perfect tool for challenging experiments like this. Applications that were impossible before turn into reality – and with its ease of use, they can also become real for your research.
Subcellular Dynamics at Highest Volume Speed
Cos 7 cells transiently transfected with CalnexinmEmerald and EB3-tdTomato. EB3 labels the growing ends of microtubules and is necessary for the regulation of microtubule dynamics. Calnexin is a protein of the ER where proteins are synthesized. Cells were imaged for 1.5 hrs every 80 secs, imaged volume: 175 × 120 × 70 μm³.
Time lapse movie showing dynamics of a U2OS cell stably expressing Actin-GFP (cytoskeleton, cyan). Cells were also labeled with MitoTracker™ Red CMXRos (Mitochondria, green) and Draq 5 (Nucleus, magenta).
Cos 7 cell transiently transfected with Tomm20-mEmerald and Calreticulin-tdTomato. Tomm20 labels the outer membrane of mitochondria, Calreticulin is a protein of the ER where proteins are synthesized. Both are extremely delicate and light-sensitive organelles that are difficult to image with conventional methods. One volume every 30 secs, imaged for 1 hr 15 mins, imaged volume: 175 × 210 × 20 μm3. A total of 85,800 images was recorded; 572 volume planes for 150 time points.
Cos 7 cell expressing Lifeact-GFP. Maximum intensity projection. The cell was imaged constantly for 9 hrs; one volume (115 × 60 × 25 μm³) every 10 secs. A total of 1,005,000 images was recorded; 201 volume planes for 5,000 time points.
Cos 7 cells transiently transfected with mEmerald-Rab5a and Golgi7-tdTomato. Golgi7 is a protein associated to the Golgi and Golgi vesicles. Rab5a is an early endosome marker. Tracking of vesicles in 3D with near-isotropic resolution becomes reality. Tracking was performed in arivis Vision4D®.
T cell expressing Lifeact-GFP. Color-coded depth projection and maximum intensity projection side- by-side. The T cell was imaged constantly for over 1 hr; one volume every 2.5 secs. Sample: courtesy of M. Fritzsche, University of Oxford, UK.
Developing Life at Early Stages | Oocytes
Fixed mouse germinal vesicle oocytes stained for the nuclear envelope (anti-lamin, cyan), actin (phalloidin, magenta), and microtubules (anti-tubulin, yellow). The Sinc3 15 × 650 lattice light sheet was used for high-resolution imaging of microtubule and actin structures. Follow the 3D structure of the microtubules in the movie. Sample: courtesy of C. So, MPI Göttingen, Germany.
Fixed mouse germinal vesicle oocytes stained for the nuclear envelope (anti-lamin, cyan), actin (phalloidin, magenta), and microtubules (anti-tubulin, yellow). The Sinc3 100 × 1,800 lattice light sheet was used for imaging of the whole oocyte. Sample: courtesy of C. So, MPI Göttingen, Germany.
Live mouse oocytes arrested in metaphase II and stained for mitochondria (cyan), microtubules (magenta) and chromosomes (yellow). Sample: courtesy of C. So, MPI Göttingen, Germany.
Developing Life of Small Evolving Organisms | Zebrafish
DeltaD-YFP transgenic zebrafish embryo (Liao et al. 2016, Nature Communications). Fusion protein driven by a transgene containing the endogenous regulatory regions, expression in the tailbud and pre-somitic mesoderm. Signal visible in the cell cortex, and in puncta corresponding to trafficking vesicles (green). Nuclei in magenta. The embryo was imaged for 5 minutes constantly; one volume (150 × 50 × 90 μm3) every 8 sec. Sample: courtesy of Prof. Andrew Oates, EPFL, Switzerland.
High-speed movie of zebrafish embryo. Volumetric imaging of trafficking mRNA molecules (green). Nuclei are shown in magenta. Data is displayed as maximum intensity projection. One volume (86 × 80 × 12 μm3) was recorded every 2.5 sec. Sample: courtesy of Prof. Andrew Oates, EPFL, Switzerland.
Trafficking mRNA molecules were tracked in arivis Vision4D®. The movement of the zebrafish embryo was first corrected using a nucleus reference track. Then individual mRNA molecules were tracked over time to result statistics such as speed and directionality. Sample: courtesy of Prof. Andrew Oates, EPFL, Switzerland.
Developing Life of Small Evolving Organisms | C. elegans Embryos
C. elegans embryo stained for nuclei. The movie shows a color-coded depth projection of the embryo. The embryo was imaged for 10+ minutes constantly; one volume every 700 msec. Imaged volume: 115 × 50 × 30 μm³. A total of 101.000 images was recorded; 101 volume planes for 1000 time points. Customer sample.
C. elegans embryo stained for nuclei. The movie shows a color-coded depth projection of the embryo. The embryo was imaged for 19+ hrs every 5 mins and can be observed going through its normal sleep-wake cycle. Imaged volume: 115 × 50 × 30 μm³. A total of 23,836 images was recorded; 101 volume planes for 236 time points. Customer sample.
C. elegans embryo at the late bean stage (~400 min post fertilization) with ~560 nuclei marked with HIS-58::mCherry (magenta) and centrioles marked by GFP::SAS-7 (green). Cells in mitosis show condensed signal of HIS-58::mCherry and centrioles at spindle poles. Sample: courtesy of N. Kalbfuss, Göncy Lab, EPFL, Switzerland.
Developing Life of Small Evolving Organisms | Drosophila Embryo
Drosophila melanogaster is a model organism in many research fields such as biomedical research. Many genetically modified variants are available to researchers. This video shows a drosophila embryo with GFP labeling as it moves over time. A total of 91,100 i mages were taken, 911 volume planes, 100 time points. One volume, every 15 secs; imaging duration 25 mins, imaging volume: 300 × 455 × 145 μm3.
Developing 3D Cell Models
Spheroids and organoids are in vitro models of organs – much smaller and simpler but easy to produce and thus for developmental biologists an invaluable tool to study organ development. Unlike cell cultures, which usually consist of a monolayer of cells only, cells in spheroids / organoids form three-dimensional structures, allowing for the investigation of cell migration and differentiation inside 3D cell models. With lattice light sheet microscopy, imaging the development and self-organization of organoids becomes reality. Here, we can see a 3D rendering of a spheroid consisting of cells expressing H2B-mCherry (cyan) and α-Tubulin-mEGFP (magenta). Not every cell is labelled.
Developing Plants and Plant Seeds | Pollen Grain & Pollen Tube
Watch mitochondrial dynamics inside the pollen tube. Mitochondria move towards the tip at the edges and back in the middle of the tube. While trafficking, mitochondria constantly fuse and divide for repair processes and to share and distribute biological molecules. Sample: courtesy of R. Whan, UNSW, Sydney, Australia.
Pollen tube stained for mitochondria (MitoTracker Green, green) and Lysosomes (Lysotracker Red, red). Watch the pollen tube extend from the crack in the pollen grain (visualized by its autofluorescence). Mitochondria don't quite advance to the very tip of the pollen tube but stop a few microns before the tip. Rendering of the data set was performed in arivis Vision4D®. Sample: courtesy of R. Whan, UNSW, Sydney, Australia.