zebrafish - ZEISS Lightsheet

Lattice-SIM

Speaker - Dr. Xianke Shi (ZEISS APAC)

• Topic: Recent advance of microscopy techniques for neuroscience research
• Abstract

Neuroscience research is one of the most fundamental life science research topic in the 21st century. Tremendous efforts have been put to understand the function of the brain from molecular, cellular and organ level. However, neuroscience is also one of the most challenging research subject, due to the enormous complexity of the nervous systems. Microscopy based visualization methods were probably the most valuable techniques for life science researchers, especially with the recent advance of fluorescence, optical sectioning, tissue clearing/expansion techniques, as well as bioscience based electron microscopy methods.
Nevertheless, visualization of the brain at different resolution level has been proven extremely demanding. Here we will brief discuss a few microscopy methods and how they can benefit neuroscience research. From low to high resolution, we will cover: lightsheet based fluorescence microscopy, Multiplex Airyscan and Lattice SIM based super-resolution microscopy, and cryo workflow for cryo TEM lamella sample preparation.

he continued his work on FCS, as well as light sheet based microscopy and in vivo single molecule detection. On January 2011, Xianke joins Carl Zeiss Microscopy, South East Asia, as a regional application specialist, and since March 2014, he is in Carl Zeiss Microscopy, Asia Pacific team.

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